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KMID : 0903619950360050730
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Journal of the Korean Society for Horticultural Science
1995 Volume.36 No. 5 p.730 ~ p.739
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Effects of Methionine , Ethionine , and DNP on the Endogenous polyamines and Acc Contents , Lioxygenase and EFE Activites , and Ethylene Production of Cut Carnation ´ Virginia ´ during Vase Life
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Abstract
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Effect of methionine, ethionine, or 2,4-dinitrophenol (DNP) added in the preservative solution on the postharvest physiology and vase life of cut carnation (Dianthus caryophyllus L.) ´Virginia´ which was revealed as a typical climacteric flower was investigated. Vase life of cut flower temporally coincided with peak time by an autocatalytic rise of ethylene regardless of treatments. Cut flowers maintained in DW and BS solution (2% sucrose + 200 ppm 8-HQS; BS), BS + methionine (0.1 mM), BS + ethionine (0.1 mM), and BS + DNP (0.1 mM) solutions showed peaks at the day of 6, 7, 7, 8, and 9 in ethylene production, respectively. It was found that ACC contents dramatically increased at the time when ethylene productions decreased in all treatments. and ethylene productions and EFE activities were delayed or inhibited in the treatments of methionine, a substrate in ethylene biosynthesis pathway and DNP as well as ethionine, inhibitor of SAM synthetase. In polyamines analyses, endogenous putrescine contents were much higher than spermidine contents regardless of treatments. Specially, putrescine contents constantly increased from the early stage of cut flower and then exactly paralleled the pattern of ethylene evolution which declined after an autocatalytic rise whereas levels of spermidines were initially declined and showed small peaks during later stages such as anthesis and induction of senescence, depending upon treatments. It was also found that levels of LOX activities paralleled the pattern described for ethylene evolution. These experimental data conclusively indicated that 1) there was no interaction between reduction of polyamines (putrescine and spermidine) contents and induction of senescence (such as ethylene evolution), 2) methionine inhibited EFE activity in the presence of ACC whereas ethionine was involved in reducing an endogenous ACC content, and finally 3) DNP was related in inhibiting EFE activity as well as producing spermidine.
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